富士山が遂に世界遺産に登録されましたね。

静岡県民としてとても嬉しいです。

梅雨が明けたら

三保の松原から富士山を眺めに行きたいと思います。

さて、一連のMTAセメントのエビデンスも今回で一段落します。

最後はラットの臼歯をMTAで直接覆髄させた文献です

This study investigated the reparative process of mechanically exposed pulps capped with mineral trioxide aggregate (MTA). Maxillary first molars of 8-week-old rats were MTA-capped for 1-14 days, and 5-bromo-2′-deoxyuridine-labeled proliferating cells and immunoreactivity for nestin and osteopontin were analyzed. MTA capping caused mild necrotic changes followed by progressive new matrix formation and calcified bridging. Proliferating cells peaked at 3 days when matrix formation was inconspicuous. Nestin-expressing cells appeared at 3 days, were arranged beneath the newly formed matrix at 5 days, and showed odontoblast-like morphology by 14 days. Osteopontin immunoreactivity was detected just beneath the necrotic area after 1 day. These findings suggest that pulpal responses to MTA capping involve proliferation and migration of progenitors followed by their differentiation into odontoblast-like cells, a mechanism basically similar to those to calcium hydroxide. Osteopontin might play a triggering role in initiation of the pulpal reparative process.

つまり

術後3日目において覆髄部近傍において

活発な細胞増殖を意味する5-bromo-2′-deoxyuridine陽性細胞が多数認められ

nestin陽性象牙芽細胞様細胞の出現が変性層下に観察された。

これらのことからMTAからの滲出物が歯髄細胞の象牙芽細胞への

分化を促していることが示唆された

ということです。

引用文献

Kuratate M, Yoshiba K, Shigetani Y, Yoshiba N, Ohshima H, Okiji T.

Immunohistochemical analysis of nestin, osteopontin, and proliferating cells in the reparative process of exposed dental pulp capped with mineral trioxide aggregate.

J Endod. 2008 Aug;34(8):970-4. doi: 10.1016/j.joen.2008.03.021. Epub  2008 Jun 20.