今回もMTADに関する文献です。

殺菌効果が高いという前回の文献に対して

今回は生体（L929線維芽細胞）への毒性を調べています。

Previous studies have shown that MTAD (a mixture of a tetracycline isomer, an acid, and a detergent) is an effective antibacterial irrigant as a final rinse to remove the smear layer from the instrumented surface of root canals. In this investigation we examined the cytotoxicity of MTAD compared with that of commonly used irrigants and medications. L929 fibroblasts were grown on cell culture plates and were placed in contact with various concentrations of test irrigants and medications. The cytotoxicity of these materials was evaluated 24 h after incubation using MTT assay. Means and standard deviations of absorbance were calculated for each group and statistically analyzed to determine presence or absence of significant difference between the means. The 50% inhibitory dose values were calculated, ranked, and statistically analyzed using the sign interval for median. Based on our results it seems that MTAD is less cytotoxic than eugenol, 3% H2O2, Ca(OH)2 paste, 5.25% NaOCl, Peridex, and EDTA and more cytotoxic than 2.63%, 1.31%, and 0.66% NaOCl.

つまり

ヒポクロ2.63％よりやや毒性が大きいですが

ユージノールやH2O2やヒポクロ5.25％よりは

毒性が小さいということです。

効果だけでなく毒性についても

別の方法で評価している文献も調べて

本当に安全に使えるものなのか判断していきたいです。

引用文献

Evaluation of cytotoxicity of MTAD using the MTT-tetrazolium method.

Zhang W, Torabinejad M, Li Y.

J Endod.2003 Oct;29(10):654-7.